Open a new Excel document or existing template. I finally found the solution in this support article from Microsoft under the 'Use a custom template for all new workbooks' section: I was struggling with this for Microsoft Excel for Mac (Office 365, Version 16.38, around May 2020). You should now find the default workbook has all the customisations you made at Steps 6 & 7. xltx extension at the end of the file name, then manually remove the. ( Note: when saving the file, if Excel has placed the. Save the workbook as an Excel template, making sure you use the name 'workbook', and make sure it's saved in the xlstart folder you created at Step 1.While you're at it, you can also customise a whole heap of other attributes if you like.Open a new workbook and customise your Zoom setting to 150%.For open all files in set the xlstart folder you created at Step 1 as the path.Go to Preferences > General > At startup.You can create this folder anywhere, but I prefer doing it within the Documents folder. On your Mac create a new folder called 'xlstart'.In the interests of providing alternatives, I thought I'd share my preferred method as well: I've also seen a number of different ways people have used to achieve a totally customised default workbook. There is a lot of confusion around how to do this, not just because it's changed from Excel 2011 to Excel 2016, but also because the process has even changed during Excel 2016's lifecycle.